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Fig. 7 | Journal of Experimental & Clinical Cancer Research

Fig. 7

From: Inhibiting PAD2 enhances the anti-tumor effect of docetaxel in tamoxifen-resistant breast cancer cells

Fig. 7

Cl-amidine combined with docetaxel synergistically decreases the activation of Akt/mTOR signaling. a Western blot analysis showing that the levels of p-Akt and p-Rps6 were decreased in MCF7/TamR cells treated with either docetaxel or cl-amidine, whereas a combination of docetaxel and cl-amidine nearly completely inhibited Akt and Rps6 phosphoryaltion. Total Akt and Rps6 proteins were not affected. GAPDH served as loading control. b MCF7/TamR cells were treated with 10 μM MHY1485, followed by cl-amidine and docetaxel treatment. Western blot analysis showing that pretreatment of cells with MHY1485 fully abolished the inhibitory effect of cl-amidine combined with docetaxel on Rps6 activation. GAPDH served as loading control. c MCF7/TamR cells were treated with 10 μM MHY1485, followed by cl-amidine and docetaxel treatment. CCK8 assay showing passively activating mTOR by MHY1485 reversed the inhibiting effect on viability of MCF7/TamR cells caused by cl-amidine and docetaxel combination treatment. (*P < 0.05). d MCF7/TamR cells were inoculated into the nude mice. Two weeks later, the mice were randomly divided into 4 groups and received PBS control, docetaxel, cl-amidine, or the combination injection (n = 6/group). The tumors were removed and the tumor weight was then recorded and plotted. *P < 0.05. e Western blot analysis of the mouse xenograft tumors with the antibodies against Bak, PCNA, p-Akt, Akt, p-Rps6, Rps6, and GAPDH

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