Skip to main content
Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: The JAK2/STAT3/CCND2 Axis promotes colorectal Cancer stem cell persistence and radioresistance

Fig. 6

Targeting CCND2 significantly sensitizes CSCs to radiation. a-g The biological effects of CCND2 knockdown were evaluated in CD44v6+ HCT116 cells with or without RT (2 Gy). a Cell cycle alteration after RT was compared between siCTRL- and siCCND2-transfected CD44v6+ cells. b Cell viability was measured by the MTT assay at 72 h after radiation treatment. c and d FACS analysis revealed (c) proliferating Ki67+ cells or (d) Annexin V+ apoptotic cells at 24 h after radiation. e FACS analysis revealed γH2AX+ DNA-damaged cells 24 h after radiation. f Comet assay visualized the extent of DNA damage accumulation 24 h after radiation. g A set of genes involved in the CCND2 downstream pathway was assessed in CD44v6+ cells transfected with siCTRL or siCCND2 by real-time qPCR. The bar graph represents the mean ± SD (n = 3). Statistical analyses were implemented using one-way ANOVA followed by Dunnett’s multiple comparison test against the control group or by Student’s t-test between two groups. The chi-squared test was used to compare the cell cycle distribution within different categories; *, **, and *** indicate p < 0.05, p < 0.01, and p < 0.001, respectively

Back to article page