Fig. 5

Mutation patterns in bladder cancer patients evaluated using Ion Ampliseq Cancer panel kit. a Bar Graph of the number of mutations identified for each gene in the Ion Ampliseq Cancer kit; b Bar Graph of the mutation frequency of each gene in the Ion Torrent Cancer panel from the analyzed UMPh patient samples; c Pie graph of the identified mutations separated by pathogenicity according to ClinVar classification; d Pie graph of the identified mutations separated by pathogenicity according to FATHMM scoring and classification; e Localization of mutations in each of the gene sequences for the most frequent mutated genes in bladder cancer patients; f qRT-PCR evaluation of TP53 gene expression level in all healthy tissues (n = 41) and bladder tumor tissues (n = 41); this cohort means the 23 samples from the UMPh patient cohort and 18 samples from the validation set. g Pirate Plot of the TP53 expression level in wild-type and TP53-mutated samples; h Kaplan-Meier curve of overall survival rate based on mutation status for TP53 mutated gene from the UMPh patient cohort; i Kaplan-Meier curve of overall survival rate based on TP53 expression levels from the UMPh patient cohort