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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Programmed cell death ligand-1-mediated enhancement of hexokinase 2 expression is inversely related to T-cell effector gene expression in non-small-cell lung cancer

Fig. 2

PD-L1 knockdown decreases the expression and activity of HK2 and glycolysis in lung cancer cells. H460 cells were transfected with scrambled control siRNA (sc), PD-L1-specific siRNA1, or 2. Forty-eight hours after transfection, mRNA and protein levels of GLUT1, HK2, and PKM2 were evaluated using qRT-PCR and Western blotting (a-d). Hexokinase activity assays (e), lactate production assays (f), and ECAR assays (g) were also performed. Histograms represent values normalized to control. Data represent the means ± SEMs of at least three independent experiments or are representative of three independent experiments. All p values were calculated using unpaired Student’s t-tests and one-way ANOVA. *p < 0.05; **p < 0.001

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