Fig. 2
Knocking down the expression of METTL3 impaired the migration and invasion ability of CRC cell lines in vitro and in vivo. (a) Left and right panel showed the expression level of METTL3 in DLD-1 and HCT116 cell lines transfected with siRNA determined by qRT-PCR and western blot. (b) Effect of knocking down the expression of METTL3 on transwell assay; Representative graphs are shown. Original magnification 200X; scale bar: 50 μm. (c) Effect of knocking down METTL3 level on scratching healing assay; The scratch was measured 48 h later; Representative graphs are shown. Original magnification, 40X; scale bar: 100 μm.(d) METTL3 level in DLD-1 cell line transfected with lentiviral shRNA. (e) Hepatic metastasis foci in metastasis model graphs were shown. Right panel showed the metastasis foci formed in the liver were compared between shControl and shMETTL3. Black dot indicated the metastatic foci. Data are presented as means ± standard deviation (*P < 0.05, **P < 0.01)