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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Upregulated METTL3 promotes metastasis of colorectal Cancer via miR-1246/SPRED2/MAPK signaling pathway

Fig. 4

Upregulated METTL3 enhanced the metastatic ability through modulating the maturation of pri-miR-1246. (a) Venn diagram showed the overlapping of metastasis-related miRNA from the top 30 ranked by P value and Log FC (GEO data, P < 0.05, Log FC > 1) (left panel); The top five miRNA were listed in the table (right panel). (b) qRT-PCR was used to measure the expression level of miR-1246 in 20 cases of primary CRC tissues with metastasis and 20 cases of primary CRC tissues without metastasis. (c) Scatter plot were showed between miR-1246 and METTL3 in CRC (r = 0.3972, *p < 0.05, n = 30). (d) Relative level of miR-1246 between tumor and adjacent normal tissues (n = 30). (e) qRT-PCR quantification of miR-1246 and pri-miR-1246 level that were affected by METTL3 depletion and overexpression. (f) Knockdown and overexpression level of miR-1246 were assessed by qRT-PCR. (g-h) The transwell migration assay in control and METTL3-depletion DLD-1 cell line or METTL3-overexpressing HCT116 cell line in the presence of miR-1246 mimics or miR-1246 inhibitors. Original magnification 200X; scale bar: 50 μm. Data are presented as means ± standard deviation (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001)

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