Fig. 6

Neutralization assay. a MV-Edm and GOS/MV-Edm were incubated with human MV immune serum (Ab) and added to HeLa or A549 cells at an MOI of 0.5. The plasma with a high neutralizing antibody titer was diluted to 1/16, followed by one-half dilutions up to 1/128. The oncolytic effect (cell death) was quantified by trypan blue exclusion in cells at 48 h postinfection. b MV-Edm-eGFP and GOS/MV-Edm-eGFP were incubated with human MV immune serum and added to HeLa cells at an MOI of 0.5. The fluorescence images of cells 60 h postinfection and GFP fluorescence intensity of three images were measured at 60 h postinfection. Mean of triplicates, # p indicates not significant, *p < 0.05, **p < 0.01. c The nude mice bearing HeLa human cervical carcinoma tumors were randomized into two groups (n = 3 for each group). On the first and fourteenth days after injection of the HeLa cells, naked MV-Edm-Luc or GOS/MV-Edm-Luc were injected peritumorally into the mice bearing HeLa tumors, and the mice had been immunized by intraperitoneal injection of 500 μl human immune serum with measles antibodies 18 h prior to injection of naked MV-Edm-Luc or GOS/MV-Edm-Luc. Two groups of mice were injected with MV-Edm-Luc or GOS/MV-Edm-Luc (2 × 10⁶ TCID50 per mouse) via the tail vein. Luciferase activity was monitored by an in vivo luminescence imaging system 72 h after virus injection (left panel). Photons per cm² of tumor were quantified (right panel). Means are shown (n = 3 of each), * p < 0.05, **p < 0.01