Fig. 1

Expression of SCP2 in human PA samples and forced expression of SCP2 promoted the proliferation of PA cell lines. a Expression of SCP2 mRNA in low proliferation index PA (LI-PA) human tissues, high proliferation index PA (HI-PA) human tissues and human pituitary gland RNA standard was assessed by qRT-PCR. b Expression of the SCP2 protein in human PA samples was assessed by IHC staining (left panel). Statistical analysis of the IHC staining (right panel). Scale bar, 50 μm. c Control (Vector) and SCP2-overexpressing (SCP2-OE) GH3 and MMQ cells were incubated with 10 μM itraconazole (ITR) for 0, 24, 48 or 72 h, and cell proliferation of the four groups (Vector, SCP2-OE, Vector + ITR and SCP2-OE + ITR) at different time points was assessed by a CCK-8 assay (left two line charts, n = 6, ± SEM). Wild-type (N) AtT-20 cells was incubated with 10 μM itraconazole (ITR) for 0, 24, 48 or 72 h, and cell proliferation of the two groups (N and N + ITR) at different time points was assessed by a CCK-8 assay (right line chart, n = 6, ± SEM). d SCP2 mRNA and protein levels were measured in GH3 cells (Vector and SCP2-OE) by RT-qPCR and Western blotting (n = 3, ± SEM). e Invasion of Vector and SCP2-OE cells was evaluated by a Transwell assay. Scale bar, 100 μm (n = 3, ± SEM). An unpaired t-test was used to assess statistical significance. *P < 0.05; **P < 0.01; ***P < 0.001; #, not significant