Fig. 1

DHA reduced viability, colony formation and induced apoptosis in glioma cells. A, Cell viability of U251 (a), U373 (b) and primary human glioma cells (named G0101 and G0107) (c) was detected by using a Promega Cell Titer 96 Aqueous One Solution at 48 h after DHA treatment at different concentrations. B, DHA inhibited the colony formation of U251 (a) and U373 (b) cells. C-E, Cell death was detected by Annexin-V/7-ADD staining assay in human primary glioma cells (C), U251 (D) and U373 (E) cells treated with different concentrations DHA for 48 h, respectively. *, P < 0.05; **, P < 0.01; ***, P < 0.001, compared to control; Data were mean ± SD from three independent experiments. n = 3 for all bar graphs