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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Dihydroartemisinin-induced unfolded protein response feedback attenuates ferroptosis via PERK/ATF4/HSPA5 pathway in glioma cells

Fig. 5

ATF4-induced HSPA5 prevented DHA-induced ferroptosis in glioma cells. A, ATF4 and xCT expression in U251 cells 24 h after DHA treatment at different concentration. The relative expression level of ATF4 standardized to β-actin was labeled. B and C, ATF4 silencing inhibited DHA-induced HSPA5 expression in glioma cells. D, HSPA5 silencing (e) enhanced DHA-induced glioma cell death (a), and ROS (b), MDA (c) and lipid ROS (d) generation. E, HSPA5 overexpression (e) inhibited DHA-induced glioma cell death (a), and ROS (b), MDA (c) and lipid ROS (d) generation. **, P < 0.01; ***, P < 0.001, compared to control. Data were mean ± SD from three independent experiments. n = 3 for all bar graphs

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