Fig. 7

HSPA5 inhibited DHA-induced ferroptosis by increasing GPX4 in glioma cells. A, GPX4 expression after HSPA5 silencing in U251 cells. B, GPX4 expression (a) and activity (b) in U251 cells treated with DHA at different concentrations. C, Inhibition of GPX4 activity by siRNA transfection (a) enhanced DHA-induced ROS (b), MDA (c) and lipid ROS (d) generation as well as cell death (e) in U251 cells. D, GPX4 overexpression (a) compensated HSPA5 siRNA-enhanced reduction of GPX4 activity (d) and abolished HSPA5 siRNA-enhanced ROS (b), MDA (c) and lipid ROS (e) generation and cell death (f) in U251 cells. *, P < 0.05; **, P < 0.01; ***, P < 0.001, compared to control. Data were mean ± SD from three independent experiments. n = 3 for all bar graphs