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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: FUBP1 promotes neuroblastoma proliferation via enhancing glycolysis-a new possible marker of malignancy for neuroblastoma

Fig. 2

FUBP1 promotes NB cell proliferation. a Protein levels of FUBP1, c-Myc and N-Myc were determined by Western blot expression in NB cells and normal control cells. β-Actin served as a loading control. b SK-N-BE (2) cells were infected with siRNA to knock down FUBP1, and SH-EP cells were infected with viruses expressing FUBP1 for 72 h, followed by CCK8 analysis. EV: Empty vector; FUBP1: Vector with FUBP1 overexpression. c SK-N-BE (2) cells were infected with siRNA to knock down FUBP1 for 72 h, followed by a monoclonal plate formation assays. d Statistical analysis of relative colony numbers in each group. e SK-N-BE (2) cells were infected with siRNA to knock down FUBP1 for 72 h, followed by EDU staining. f SH-EP cells were infected with siRNA to knock down FUBP1 for 72 h, followed by EDU staining. Scale bar, 100 μm. Error bars represent the standard deviation (SD); two asterisks, p < 0.01; asterisks, p < 0.001

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