Fig. 4

FUBP1 promotes NB cell growth through glycolysis. SK-N-BE (2) cells were stably infected with lentiviral containing shRNA specific to FUBP1 gene (shFUBP1) or shRNA containing scramble sequences (shCON) for 48 h. SH-EP cells were transfected with plasmid containing over-expressing FUBP1 gene sequences (FUBP1) or empty vectors (EV) for 48 h. The levels of FUBP1 levels in shFUBP1 or plasmid overexpressing FUBP1 cells were determined by Western blot. β-Actin served as a loading control. (a) SK-N-BE (2) cells were treated with 2-DG (2 mM) for 72 h, followed by CCK8 analysis. (b) SH-EP cells were treated with 2-DG (2 mM) for 72 h, followed by CCK8 analysis. (c) SK-N-BE (2) cells were treated with 2-DG (2 mM) for 72 h, followed by glucose consumption using a glucose assay kit. (d) SH-EP cells were treated with 2-DG (2 mM) for 72 h, followed by glucose consumption using a glucose assay kit. (e) Cell ATP production analysis. (f) Extracellular oxygen consumption analysis. (g) Statistical analysis of oxygen consumption analysis. (h) Lactate analysis in cell supernatant. (i) Lactate analysis in NB tissues (GNB: n = 6; NB: n = 10). (j) LDH activity analysis in cells. (k) LDH activity analysis in NB tissues (GNB: n = 6; NB: n = 10). Error bars represent the standard deviation (SD); one asterisk, p < 0.05; two asterisks, p < 0.01; asterisks, p < 0.001; NS means no significant difference