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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: FUBP1 promotes neuroblastoma proliferation via enhancing glycolysis-a new possible marker of malignancy for neuroblastoma

Fig. 5

FUBP1 was positively correlated with the expression of HIF1α and LDHB. (a) qPCR analysis of glycolytic key enzymes genes expression, including: Hk2, G6pdh, Fbp1, Pfk1, Pkm, Ldha, Ldhb in the SH-EP cell, followed by transfecting with plasmid overexpressing FUBP1 or with siRNA to knock down FUBP1 was shown. (b) Immunohistochemistry staining of LDHB on NB TMA tissue. (c) Correlation analysis of LDHB and FUBP1 expression in NB TMA tissue. (d) Statistical analysis of the histochemistry score (H-Score) of LDHB in TMA. (e) Survival of NB patients with high expression of LDHB (H-Score ≥ 5) versus those with low expression of LDHB (H-Score < 5). (f) Western blot analysis of HIF1α and LDHB expression in frozen peripheral neuroblastic tumour (pNT) tissue samples (5 NB, 5 GNB, 2 GN). β-Actin served as a loading control. (g) The correlation analysis of FUBP1 and HIF1α expression in NB frozen tissues. (h) Western blot analysis of HIF1α and LDHB expression in NB cells and normal control cells. β-Actin served as a loading control. Error bars represent the standard deviation (SD); one asterisk, p < 0.05; asterisks, p < 0.001

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