Fig. 1

Up-regulated CyclinD1 is associated with down-regulated Dicer expression and poor survival in patients with ICC. a-b The levels of CyclinD1 and Dicer in 27 primary ICC and 9 adjacent non-tumor tissues were examined by IHC. Scale bar, 500 μm (upper panel, red line) and 20 μm (low panel, red line) respectively. b The CyclinD1 expression (upper panel) and Dicer expression (low panel) were examined by semi-quantitatively analyzed. c The levels of CyclinD1 and Dicer mRNA transcripts in 14 pairs of ICC and adjacent non-tumor tissues were determined by quantitative RT-PCR and the coefficient was examined by Pearson correlation (r = − 0.964, **p < 0.01). d Western blot analysis of the relative levels of CyclinD1 and Dicer to Histone3 expression in five pairs of ICC and adjacent non-tumor tissues. e Kaplan–Meier analysis of the overall survival of 33 ICC patients with high CyclinD1(n = 21) and lower Dicer (n = 22) expression vs. patients with lower CyclinD1 (n = 12) and higher Dicer (n = 11) expression. Data are representative images or expressed as individual mea values or the means ± SD of each group from three separate experiments. *p < 0.05, **p < 0.01, ***p < 0.001