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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: CyclinD1 inhibits dicer and crucial miRNA expression by chromatin modification to promote the progression of intrahepatic cholangiocarcinoma

Fig. 2

CyclinD1 silencing enhances Dicer expression while Dicer over-expression reduces CyclinD1 expression in ICC cells. a-b The relative levels of CyclinD1 and Dicer expression were determined respectively in ICC and non-tumor HIBEpic cells by quantitative RT-PCR and Western blot. c-d CyclinD1 silencing and related Dicer expression was demonstrated in HuccT-1, HCCC9810 and HIBEpic cells by quantitative RT-PCR and Western blot. e-f Dicer over-expression and CyclinD1 expression was demonstrated in HuccT-1, HCCC9810 and HIBEpic cells by quantitative RT-PCR and Western blot. g Western blot demonstrate that CyclinD1 silencing has no relation with expression two essential components of miRNA processing complex Ago2 and Drosha in HuccT-1, HCCC9810 and HIBEpic cells. Data are representative images or expressed as individual mea values or the means ± SD of each group from three separate experiments. *P < 0.05, **p < 0.01, ***p < 0.001

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