Fig. 6

Dicer silencing reduces miR-1914-3p/5p and miR-541-3p/5p expression, leading to increased levels of CyclinD1 and CDK6 expression in ICC cells. a Microarray analysis of 17 miRNAs in ICC and control cells. b Quantitative RT-PCR analysis of miR-1914-3p/5p and miR-541-3p/5p expression in LV-siR-Dicer or Lv-saR-CyclinD1 and control of HuccT-1 and HCCC9810 cells. c Western blot analysis of Dicer/CDK6/CyclinD1 expression in in LV-siR-Dicer and control HuccT-1 and HCCC9810 cells. d Schematic illustration of the potential biding motifs for miR-1914-5p and miR-541-5p in the 3′-UTR of CDK6 and CyclinD1 and their mutants. e miR-1914-5p and miR-541-5p reduce the CyclinD1 and CDK6 3’UTR-regulated luciferase activity in HEK239T cells. f Western blot analysis reveals that knockdown of endogenous miR-1914-5p or miR-541-5p increases CyclinD1 and CDK6 expression in HuccT-1 and HCCC9810 cells. Data are representative images or expressed as individual mea values or the means ± SD of each group from three separate experiments. *P < 0.05, **p < 0.01, ***p < 0.001