Fig. 2

The expression of UFM1 was negatively correlated with the aggressive behaviors of gastric cancer cells in vitro and in vivo. a Wound-healing assays of stably down-regulated UFM1 expression in HGC-27 and AGS cells were performed. The representative images and the quantification were presented (*P < 0.05). b Migration assays of stably down-regulated UFM1 expression in HGC-27 and AGS cells were performed. The representative images and the quantification of the results are presented as mean ± SD; scale bar, 50 μm (* P < 0.05). c Invasion assays of stably down-regulated UFM1 expression in HGC-27 and AGS cells were performed. The representative images and the quantification of the results are presented as mean ± SD; scale bar, 50 μm (* P < 0.05). d Wound-healing assays of stably up-regulated UFM1 expression in HGC-27 and AGS cells were performed. The representative images and the quantification was presented (*P < 0.05). e Migration assays of stably up-regulated UFM1 expression in HGC-27 and AGS cells were performed. The representative images and the quantification of the results are presented as mean ± SD; scale bar, 50 μm (* P < 0.05). f Invasion assays of stably up-regulated UFM1 expression in HGC-27 and AGS cells were performed. The representative images and the quantification of the results are presented as mean ± SD; scale bar, 50 μm (* P < 0.05). g Gross photos of flank xenografts and microscopic photo leading edge of tumors. h Representative images of whole livers showing reduction in UFM1 liver tumour burden after infection with UFM1 compared to infection with control and showing a significant reduction in tumour burden after infection with UFM1 compared to control (n = 5 mice). **P < 0.01, two-sided unpaired t-test. Data are mean ± SD