Fig. 3

UFM1 suppresses the metastatic potential and epithelial-to-mesenchymal transition of gastric cancer. a Representative images of cell morphology in stable HGC-27 and AGS cell expressing control shRNA (shNC) or UFM1 shRNA; scale bar, 50 μm. b Representative images of cell morphology in stably down-regulated UFM1 expression in AGS cell by immunofluorescence staining. c The lysates of stably down-regulated UFM1 expression in HGC-27 and AGS cells were respectively applied to western blot to detect the expression levels of E-cadherin, N-cadherin, vimentin, Snail and UFM1. d The lysates of stably up-regulated UFM1 expression in HGC-27 and AGS cells were respectively applied to western blot to detect the expression levels of E-cadherin, N-cadherin, vimentin, Snail and UFM1. e Stable HGC-27 cells were applied to real-time PCR. Data shown were the log values of the fold-changes in mRNA levels as compared to control. f Stable AGS cells were applied to immunofluorescence staining. Images shown were representatives in each group