Fig. 4

UFM1 suppresses epithelial-to-mesenchymal transition of gastric cancer through inactivating AKT/GSK-3β pathway. a The lysates of stable AGS cells were applied to Phospho-Kinase Antibody Array, and pixel densities of indicated proteins were shown in the right panel. b The lysates of stable AGS cells were applied to western blot to detect the expression levels of P110, P85, p-AKT, AKT, p-GSK3βand GSK3β. c The effect of UFM1 stable expression on AGS and HGC-27 cell migration was rescued by LY294002. d Quantitative results of (c) is show. The data are presented as the mean ± SD; scale bar, 50 μm (**, P < 0.01; ns, no significance). e Stable AGS cells were treated with DMSO or LY294002. Then cell lysates were applied in western blot analysis