Fig. 2

Knockdown MEIS2C/D inhibited growth and metastasis of HCC cells in vitro. a, b Expression of MEIS2C/D were measured by qRT-PCR and western blot in HCC-LM3 and MHCC97H cells treated with shMEIS2C/D targeting MEIS2C/D (exon 13) or Scramble. c, d The in vitro proliferation function of MEIS2C/D was measured by the CCK-8 Kit and colony-forming assay. The cells were seeded in 6-well plates. e, f Migration and invasion were measured by Transwell assay coated with or without Matrigel in the indicated stable HCC cell lines. HCC cells were seeded in 12-well plates. Representative images are shown (scalebar 100 μm). Experiments were repeated three times with similar results, and error bars represent the mean ± SEM, *P < 0.05, **P < 0.001