Fig. 2

PLK3 suppresses proliferation and glucose metabolism of CRC cells. a Cell proliferation as measured by EdU assay was inhibited by PLK3 overexpression in SW480 cells and promoted by PLK3 knockdown in HCT116 cells. Scar bars, 200 μm. b CCK-8 assay showing PLK3 overexpression reduces cells viability of SW480 and PLK3 depletion enhances the viability of HCT116 cells. c Morphological observation of formed xenografts, the volumes measured every 6 days and average weight of tumors. d Immunoblotting analysis of PLK3 and PCNA in tumor samples. e Lactate production and glucose consumption of SW480 and HCT116 cells were determined. f Analysis of ECAR of PLK3-overexpressing SW480 cells and HCT116 cells with PLK3 knockdown. g Analysis of OCR of PLK3-overexpressing SW480 cells and HCT116 cells with PLK3 knockdown. h ATP production was examined in SW480 and HCT116 cells. Data represent the mean ± SD of at least three independent experiments. **P < 0.01, ***P < 0.001