Fig. 3

HK2 plays a role in PLK3-mediated CRC cells glycometabolism. a A total of 84 genes are quantified using a human glucose metabolism PCR Array. The color scheme represents gene expression changes on a log2 scale. b Cells transfected with pGLV-PLK3 and sh-PLK3, respectively, were subject to immunoblotting. c Immunoblotting analysis of HK2 in SW480 and HCT116/sh-PLK3 cells with HK2 depletion. d Lactate production and glucose consumption were determined in SW480 and HCT116/sh-PLK3 cells with HK2 knockdown. e Analysis of ECAR of SW480 and HCT116/sh-PLK3 cells with HK2 inhibition. f Analysis of OCR of SW480 and HCT116/sh-PLK3 cells with HK2 inhibition. g ATP production was examined in SW480 and HCT116/sh-PLK3 cells with HK2 knockdown. h Representative images of HK2 positive and negative staining in the tissue microarray. i Expression correlation of PLK3 and HK2 was analyzed in 116 CRC patients using IHC. Data represent the mean ± SD of at least three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001