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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Polo-like kinase 3 inhibits glucose metabolism in colorectal cancer by targeting HSP90/STAT3/HK2 signaling

Fig. 4

STAT3 is involved in the regulation of HK2 by PLK3 in CRC cells. a Immunoblotting analysis of HIF-1α, c-Myc, p-STAT3 (Ser727), p-STAT3 (Tyr705), total STAT3, p-p65 and MZF1 in PLK3-overexpressing SW480 cells and PLK3-depleted HCT116 cells. b Immunoblotting analysis of p-STAT3 (Ser727), total STAT3 and HK2 in SW480 and HCT116/sh-PLK3 cells with STAT3 knockdown. c The HK2 promoter luciferase construct (− 2131/− 1505) HK2 was co-transfected with si-STAT3, and promoter activities were detected. d WT or Mutated HK2 promoter constructs were co-transfected with si-STAT3, and luciferase activities were determined. The schematic constructs are shown (left), and the bar graphs present the relative luciferase activity in each sample (right). e ChIP assays of STAT3 occupancy on the HK2 promoter. The level of STAT3 binding site in SW480/si-NC cells and normal tissues was set to 1.0, and that in others was normalized relative to the control. Data represent the mean ± SD of at least three independent experiments. **P < 0.01, ***P < 0.001

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