Fig. 5

PLK3-medatied HSP90 plays a crucial role in glucose metabolism of CRC cells. a SW480 and HCT116 cells transfected with pGLV-PLK3 and sh-PLK3, respectively, were determined by immunoblotting for PLK3 and HSP90. MG-132 (5 μg/ml) was used to treat cells for 4 h before harvesting. Densitometry represents the expression of the proteins relative to GAPDH. b PLK3 overexpression or knockdown did not change HSP90 mRNA expression levels, detected by qPCR. c Immunoblotting analysis of HSP90, p-STAT3 (Ser727) and HK2 in SW480 and HCT116/sh-PLK3 cells with HSP90 silencing. d HSP90 was co-immunoprecipitated with PLK3 and STAT3 in SW480 cells with and without HSP90 depletion. e Lactate production and glucose consumption were determined in SW480 and HCT116/sh-PLK3 cells with HSP90 knockdown. f Analysis of ECAR of SW480 and HCT116/sh-PLK3 cells with HSP90 inhibition. g Analysis of OCR of SW480 and HCT116/sh-PLK3 cells with HSP90 inhibition. h ATP production was examined in SW480 and HCT116/sh-PLK3 cells with HSP90 knockdown. Data represent the mean ± SD of at least three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001