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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: Polo-like kinase 3 inhibits glucose metabolism in colorectal cancer by targeting HSP90/STAT3/HK2 signaling

Fig. 6

miR-106b inhibits PLK3 expression by targeting the 3′-UTR of PLK3 mRNA. a A schematic diagram of the protocol used to search for miRNAs that target PLK3. b miR-106b mimics significantly reduced PLK3 mRNA expression, determined by qPCR. c Immunoblotting analysis of PLK3 in SW480/PLK3 and HCT116 cells transfected with miR-106b or anti-miR-106b. Densitometry represents the expression of the protein relative to GAPDH. d TOP, predicted miR-106b binding site in the 3′-UTR of PLK3 mRNA; bottom, sequences of wild-type and mutated PLK3 3′-UTR-Renilla luciferase reporters. e Relative activity of luciferase reporters with PLK3 3′-UTR after co-transfection with miR-106b mimics in 293 T cells. f Model of PLK3 as a key regulator that orchestrates glucose metabolism in colorectal cancer through the HSP90/STAT3/HK2 signaling. Data represent the mean ± SD of at least three independent experiments. **P < 0.01, ***P < 0.001

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