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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Necroptosis regulates tumor repopulation after radiotherapy via RIP1/RIP3/MLKL/JNK/IL8 pathway

Fig. 2

Nec-1 inhibits radiation-induced necroptosis rather than apoptosis in vitro. (a-d) The colorectal cancer cell lines (HT29, SW480 and HCT116) were pre-treated with or without Nec-1 for 24 h prior to radiation. a Clonogenic survival assays of irradiated HT29, SW480 and HCT116 cells were performed as indicated, student’s t-test, n = 3. b Representative graphs and statistical analysis of flow cytometry using Annexin V/PI double staining showed the cell death manner of HT29, SW480 and HCT116 after 0 Gy or 10 Gy irradiation. Percentage decreased PI positive cells by Nec-1counted as necroptosis, one-way ANOVA, n = 3. c LDH was quantified in cell culture supernatants of HT29, SW480 and HCT116 with or without 10 Gy radiation, one-way ANOVA, n = 3. d Cell viability of HT29, SW480 and HCT116 cells were assessed using a CCK-8 assay with no irradiation (upper panel) or after 10 Gy radiation (lower panel), student’s t-test, n = 3. n.s = not significant, * < p 0.05, ** p < 0.005, *** p < 0.001

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