Fig. 4

Blockade of the RIP1/RIP3/MLKL signaling pathway in irradiated tumor cells attenuated the proliferation of living tumor cells nearby in vitro. a 10Gy-irradiated tumor cells prompted the proliferation of living tumor cell. Upper panel, growth of HT29 Fluc or SW480 Fluc were observed by luciferase activity imaging. Lower panel, representative bioluminescence images, one-way ANOVA, n = 3. b Proliferation-promoting effect of dying HT29 and SW480 cells on HT29 Fluc and SW480 Fluc in vitro. The dying cells pretreated with RIP1 inhibitor (Nec-1), RIP3 inhibitor (GSK’872) or MLKL inhibitor (NSA) for 24 h, one-way ANOVA, n = 3. c Proliferation-promoting effect of dying sh-RIP1 HT29 cells, sh-RIP3 HT29 cell and sh-MLKL HT29 cells on HT29 Fluc cells, compared with equally treated vector-transfected HT29 cells, one-way ANOVA, n = 3. n.s = not significant, ** p < 0.005, *** p < 0.001