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Table 2 Antibodies for Western blot (WB), immunohistochemistry (IHC) and immunofluorescence (IF) analyses

From: Impact of Docetaxel on blood-brain barrier function and formation of breast cancer brain metastases

Antibody

Company

Catalog #

Dilution (WB/ IHC/ IF)

α-tubulin

Sigma-Aldrich

T6199

1:1000/ X/ X

β-tubulin

Abcam

ab6046

X/ X/ 1:200

β-actin

Abcam

ab8227

1:1000/ X/ X

Claudin-5 (rb)

Thermo Fisher Scientific

34–1600

1:500/ 1:200/ 1:200

Claudin-5 (ms)

Invitrogen

35–2500

X/ X/ 1:200

Zonula-Occludens-1

Thermo Fisher Scientific

61–7300

1:500/ 1:500/ 1:200

Occludin

Thermo Fisher Scientific

71–1500

2 μg/mL/ 1:50/ X

Occludin

Invitrogen

OC-3F10

X/ X/ 1:200

VE-Cadherin

Santa Cruz

Sc-6458

1:500/ 1:200/ 1:200

Tie-2

R&D

AF762

0.1 μg/mL/ 1:200/ X

pTie-2

R&D

AF2720

0.5 μg/mL/ 2.5 μg/mL

ABCC4

Cell signaling

12857S

1:1000/ X/ X

wide-spectrum CK

Abcam

ab9377

X/ 1:20/ X

anti-mouse IgG

Dianova

115–035-146

X/ 1:500/ X

GFAP

Dako

ZO334

X/ X/ 1:5000

Iba1

Wako

019–19,741

X/ X/ 1:500

Ang2

Thermo Scientific

PA5–27297

1:5000/ 1:800/ X

CD31 (rt)

Dianova

DIA-310

X/ X/ 1:200

DAPI

invitrogen

D1306

X/ X/ 1:1000

  1. ms mouse, rb rabbit, rt rat