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Table 2 Antibodies for Western blot (WB), immunohistochemistry (IHC) and immunofluorescence (IF) analyses

From: Impact of Docetaxel on blood-brain barrier function and formation of breast cancer brain metastases

Antibody Company Catalog # Dilution (WB/ IHC/ IF)
α-tubulin Sigma-Aldrich T6199 1:1000/ X/ X
β-tubulin Abcam ab6046 X/ X/ 1:200
β-actin Abcam ab8227 1:1000/ X/ X
Claudin-5 (rb) Thermo Fisher Scientific 34–1600 1:500/ 1:200/ 1:200
Claudin-5 (ms) Invitrogen 35–2500 X/ X/ 1:200
Zonula-Occludens-1 Thermo Fisher Scientific 61–7300 1:500/ 1:500/ 1:200
Occludin Thermo Fisher Scientific 71–1500 2 μg/mL/ 1:50/ X
Occludin Invitrogen OC-3F10 X/ X/ 1:200
VE-Cadherin Santa Cruz Sc-6458 1:500/ 1:200/ 1:200
Tie-2 R&D AF762 0.1 μg/mL/ 1:200/ X
pTie-2 R&D AF2720 0.5 μg/mL/ 2.5 μg/mL
ABCC4 Cell signaling 12857S 1:1000/ X/ X
wide-spectrum CK Abcam ab9377 X/ 1:20/ X
anti-mouse IgG Dianova 115–035-146 X/ 1:500/ X
GFAP Dako ZO334 X/ X/ 1:5000
Iba1 Wako 019–19,741 X/ X/ 1:500
Ang2 Thermo Scientific PA5–27297 1:5000/ 1:800/ X
CD31 (rt) Dianova DIA-310 X/ X/ 1:200
DAPI invitrogen D1306 X/ X/ 1:1000
  1. ms mouse, rb rabbit, rt rat