Fig. 3

FL3 reduces ERK–MNK–eIF4E signaling pathway in DLBCL cell lines. a Schematic representation of the FL3 mechanism in disrupting PHB-C-Raf interaction and thus in C-Raf-MEK-ERK signaling pathway. b ERK1/2 (ERK) and phospho-ERK1/2 (p-ERK) expression determined by Western blotting after 72 h treatment (FL3, 1–20 nM) as compared to control (C, DMSO control) in GCB (SUDHL4, SUDHL6) and ABC (OCI-LY3, OCI-LY10) cell lysates. Densitometry analysis of phospho-ERK to total ERK levels is shown for SUDHL4 and OCI-LY3 and data were expressed as the means ± SD of three independent experiments. Actin was used as a loading control. *, ** indicate significant differences to control with p < 0.05 and p < 0.01 respectively. c Immunoblot analysis of phospho-MNK1, MNK1, phopho-eIF4E and eIF4E protein levels in SUDHL4 and OCI-LY3 cell lysates representative of 3 independent experiments. d SUDHL4 and OCI-LY3 cell lines were cultured with or not (C, DMSO control) FL3 (1–20 nM). Representative immunoblots of total and phospho-eIF4E protein levels of three independent experiments are shown. Histograms show quantification by densitometry of phospho-eIF4E normalized to total eIF4E levels. Data are the means ± SD. ** p < 0.01 compared to control