Fig. 4

FL3 disrupts eIF4F complex and inhibits cap-dependent translation in DLBCL cell lines. a SUDHL4 and OCI-LY3 cells were cultured with or without FL3 (20 nM). Protein lysates from cell cultures were harvested following 72 h treatment and incubated with m7GTP agarose beads overnight at 4 °C. Western blot analysis was performed to observe eIF4A and eIF4E binding to m7GTP in eluates from beads (m7GTP pull-down) and total extracts (input). Data are representative of two independent experiments. b Immunoblot analysis of Bcl-2 and c-Myc protein levels in SUDHL4 and OCI-LY3 cell cultures in the presence or not to FL3 (1–20 nM). Actin was used as a loading control. Densitometric analysis of Bcl-2 and c-Myc levels normalized to actin is shown relative to control. Results are presented as means ± SD of 4 independent experiments. *p < 0.05, **p < 0.01 vs control