Fig. 5

FL3 decreases Akt protein level in DLBCL cell lines. a Cell lysates from FL3-treated SUDHL4 and OCI-LY3 cell lines were subjected to Western blot analysis of total (Akt) and phospho-Ser473-Akt (p-Akt) protein level. Actin is shown as a loading control. Results are representative of three independent experiments. b A concentration-dependent effect of FL3 on Akt protein level was observed in all GCB and ABC DLBCL cell lysates. c SUDHL4 and OCI-LY-3 cells were treated for 24, 48 and 72 h with or without 20 nM of FL3 and Akt protein levels were analyzed by Western blot. d Relative quantification by qRT-PCR analysis of AKT1 and AKT2 mRNA in SUDHL4 and OCI-LY3 cell cultures in presence or not of FL3 (20 nM). Results are expressed relative to control cultures as the means ± SD of six independent experiments. ***: p < 0.001 respectively vs control. e The decrease in Akt protein level was partially prevented by 2 h pre-treatment of SUDHL4 and OCI-LY3 cells with the pan-caspase inhibitor, z-VAD-fmk (80 μM), before 24 h exposition with FL3 (20 nM). Apoptosis was evaluated by Western blot analysis of PARP cleavage in cell lysates. Data are representative of two independent experiments. FL: full-length (FL), CL: cleaved PARP. Actin was used as a loading control