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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: DNMT3b/OCT4 expression confers sorafenib resistance and poor prognosis of hepatocellular carcinoma through IL-6/STAT3 regulation

Fig. 3

p-STAT3 activation enhances the expression of DNMTs/OCT4 and increases ALDH activity in Hep3B cells. The expression levels of DNMTs and OCT4 in Hep3B overexpressed with STAT3-WT, STAT3-Y640F (constitutively phosphorylated) or STAT3-Y705F (dominant negative). a Western blot analysis. b The quantitative data of (a). *P < 0.05, **P < 0.01, ***P < 0.001, ***P < 0.0001, student t test. c ALDH activity in Hep3B cells transfected with STAT3-WT, STAT3-Y640F or STAT3-Y705F. d The quantitative data of (c). *P < 0.05, student t test. ALDH, aldehyde dehydrogenase; DEAB, diethylaminobenzaldehyde; SSC, side scatter. e The protein levels of DNMT3b and OCT4 in STAT3-Y640F-Hep3B cells with or without RNA silencing of DNMT3b or DNMT1. shCtrl, control vector; shDNMT3b, DNMT3b silencing plasmid; and shDNMT1, DNMT1 silencing plasmid. e Western blot analysis. f The quantitative data of (e). *P < 0.05, **P < 0.01, student t test

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