Fig. 6

Combination of nanaomycin A increases the sorafenib susceptibility in Hep3B sorafenib resistant HCC cells. (a) The cell viability of Sorafenib-naïve Hep3B cells or sorafenib-resistant Hep3B cells under Sorafenib treatment (0, 1, 5, 10, 15 20 μM) (b) The mRNA levels of IL-6R, stemness-related genes, and DNMTs in Hep3B naïve/resistant cells were shown. Real-time Q-PCR assay. *P < 0.05, **P < 0.01, ***P < 0.001, student t test. (c, d) The cell viability of Hep3B naïve/resistant cells under the treatment of Sorafenib (1, 5, 10, 15 20 μM) with or without nanaomycin A (2, 5, 10 μM) for 48 h. The WST assay. *P < 0.05, **P < 0.01, ***P < 0.001, student t test. e The calculated combination index (CI) values of the (c, d) were shown. The meaning of CI was interpreted as: CI > 1, antagonistic effect; CI = 1, additive effect; and CI <  1, synergistic effect