Fig. 4

NT1721 induced tumor suppressor genes and decreased the invasive potential of pancreatic cancer cell lines. a NT1721-mediated induction of tumor suppressor genes (BIM, p21) and CDK2 was assessed by Western blot after 24 h using GAPDH as loading control. b CDK2 mRNA expression was determined after 24 h treatment of Panc1 and Capan1 cells with NT1721; GAPDH was used as reference gene. The graphs represent the mean ± SD from 4 independent experiments. c PDAC cell lines were treated with NT1721 for 16 h. Their invasive potential was quantified using the CytoSelect™ 96-Well Cell Invasion Assay (Cell Biolabs). d qPCR to determine ZEB1 and ZEB2 mRNA expression levels in PDAC cells and non-tumorigenic HPDEC cells. GAPDH was used as reference gene. The graphs represent the mean ± SD from 4 experiments. e Protein expression of EMT markers (ZEB1, ZEB2, CDH2, MMP2) was determined by Western blot in Panc1 and Capan1 cells after 48 h treatment with NT1721. f The mRNA expression of EMT markers (MMP2, CDH2) was assessed in Panc1 and Capan1 cells after 24 h treatment with NT1721