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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: Proteasomal cysteine deubiquitinase inhibitor b-AP15 suppresses migration and induces apoptosis in diffuse large B cell lymphoma

Fig. 1

b-AP15 induces cell apoptosis and inhibits cell proliferation in two types of DLBCLs, ABC and GCB. (a) b-AP15 decreases cell viability of SU-DHL-2, SU-DHL-4, OCI-LY-1, OCI-LY-19 cells. DLBCL cells were cultured with b-AP15 at various concentrations for 48 h, and then were subjected to MTS assay. Mean ± SD(n = 3)(b) The cell viability of PBMCs from normal donors treated with b-AP15over 48 h. Mean ± SD(n = 3)(c) b-AP15 inhibits cell proliferation in both ABC and GCB-DLBCL cells. SU-DHL-2, SU-DHL-4, OCI-LY-1, OCI-LY-19 cells were planted in 24- well plates and treated with different doses of b-AP15 for 6 h, 12 h, 24 h. Then total cell number was detected by trypan blue exclusion staining. Mean ± SD (n = 3). (d) b-AP15 induces apoptosis in GCB- and ABC-DLBCL cells. SU-DHL-2, SU-DHL-4, OCI-LY-1, OCI-LY-19 cells were treated with showed concentrations for 24 h and apoptotic cells were detected by Annexin V-FITC / PI double staining, the images shown the Annexin V+/ PI+cells captured with inverted fluorescence microscope, and cell apoptosis was detected by flow cytometryexhibited by four-quadrant diagrams. The graphs were the statistics of the flow cytometry assay. Mean ± SD (n = 3). *P < 0.05, **P < 0.01, versus control group

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