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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Proteasomal cysteine deubiquitinase inhibitor b-AP15 suppresses migration and induces apoptosis in diffuse large B cell lymphoma

Fig. 5

b-AP15 mediated down-regulation of molecules which were associated with progression in both GCB-and ABC-DLBCL cells. a b-AP15 decreases protein levels of c-Myc, NF-κB and phosphorylated NF-κB. Western blot analysis of DLBCL cells treated with b-AP15 as indicated both dose- and time-dependently was performed. b b-AP15 decreases mRNA expression of Bcl-2, c-Myc and NF-κB. SU-DHL-4 and SU-DHL-2 cells were treated for 3, 6 and 12 h with exposure to 0.1 μM and 0.5 μM b-AP15 respectively. The Bcl-2, c-Myc and NF-κB mRNA expression were measured by RT-qPCR and its expression level relative to the control was calculated. Mean ± SD (n = 3). *P < 0.05, **P < 0.01, ***P < 0.0001, versus control group. c b-AP15 decreases the protein levels of cell growth related signaling pathway AKT and STAT5. SU-DHL-4 and SU-DHL-2 cells weretreated with various concentrations or different duration of b-AP15. The total and phosphorylated AKT and STAT5 were analyzed by Western blot. Representative images were shown

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