Fig. 5

NOS1 promotes melanoma lung metastasis by S-nitrosylation of HDAC2-C262/274. a The mRNA expression of HDACs (1, 2, 3) and protein expression of HDAC2 in HDAC2-KO cells were determined by RT-PCR and western blotting. b The same method as a was used to detect the mRNA and protein expression levels of HDAC2 of stably transfected HDAC2-WT and HDAC2-MUT plasmids in HDAC2-KO cells. c-e 3 × 10⁶ B16-WT, B16-HDAC2-KO, B16-HDAC2-WT, B16-HDAC2-MUT cells were intravenously injected into C57BL/6 mice. The mice were sacrificed on the 11th day after tumor cell inoculation. Tumor growth was monitored by c gross morphology, d lung weight (n = 4), and e survival rate (n = 8). ns, not significant; **p < 0.01; ***p < 0.001; ****p < 0.0001