Fig. 6

Depletion of MYO1C enhances CEP-inhibited the interaction/ colocalization of MYO1C/F-action with LC3 and LAMP1. a MDA-MB-231 cells were transfected with control siRNA (shCon) and MYO1C siRNA (shMYO1C); the expression of MYO1C was determined by western blot. b shCon and shMYO1C cells were treated without or with CEP (4 μM) or Rapa (0.25 μM) for 24 h. Whole-cell lysate was prepared and subjected to immunoprecipitation using anti-LC3 and the associated MYO1C and actin were determined using immunoblotting. c The fluorescent images of both shCon and shMYO1C cells immunostained for MYO1C (blue), F-actin (green) and LC3 (red) The number of LC3 puncta colocalized with MYO1C and F-actin was quantified from 50 cells in three dependent experiments, data was present as mean ± SD (n.s, not significant; *P < 0.05; **P < 0.01). d LAMP1 was immunoprecipitated, and the expressions of MYO1C and actin were determined by immunoblotting. e The fluorescent images of both shCon and shMYO1C cells immunostained for MYO1C (blue), F-actin (green) and LAMP1 (red). The number of LAMP1 puncta colocalized with MYO1C and F-actin was quantified from 50 cells in three dependent experiments, data was present as mean ± SD (n.s, not significant; *P < 0.05; **P < 0.01)