Fig. 9

Overexpression of MYO1C promotes the interaction/colocalization of LC3 and LAMP1, and reduced the accumulation of mitophagosomes. The vector and MYO1C cells were treated without or with CEP (4 μM) or Rapa (0.25 μM) for 24 h. a Whole-cell lysate was prepared and subjected to immunoprecipitation using anti-LC3 and the associated LAMP1 was determined using immunoblotting. b The fluorescent images showed the colocalization of LC3 (red) and LAMP1 (green). The Pearson’s correlation coefficient (R²) of LC3 and LAMP1 colocalization was from 50 cells of three independent experiments (mean ± SD, n.s, not significant; *P < 0.05; **P < 0.01). c The mitochondrial fraction was prepared and subjected to western blot using antibody against LC3. The ratio of LC3-II/LC3-I was quantified in three independent experiments (mean ± SD, n.s, not significant; *P < 0.05 or **P < 0.01). d The fluorescent images of both vector and MYO1C cells transfected with EGFP-LC3 (green) and RFP-Mito (red). Quantification of EGFP-LC3 and RFP-mito colocalization puncta in 50 cells of three independent experiments (n.s, not significant; **P < 0.01)