Fig. 3

ILF3 is positively regulated by ERp57. a SW839 cells transfected with shERp57 or A498 cells transfected with pWP-ERp57. Gene mRNA level was tested by qRT-PCR. *P < 0.05 vs. corresponding control. b Cells were prepared as A, IL3 protein level was measured by western blotting. c ILF3 mRNA level in ccRCC tissues (n = 35) and normal kidney tissues (n = 35) was detected by qRT-PCR. d ILF3 level from ccRCC tissues and normal kidney tissues was analyzed in TCGA database. e and f Western blot analysis and immunohistochemistry stain were performed to measure the ILF3 protein level in ccRCC tissues and normal kidney tissues. g Kaplan–Meier analysis was used to analyze the overall survival of ccRCC patients with low or high ILF3 level from TCGA database. h Pearson correlation analysis was used to analyze the relationship between ILF3 and ERp57 (R = 0.7351, P < 0.0001). i Immunofluorescence staining was used to locate and detect the expression of ERp57 and ILF3 in A498 cells. Scale bars = 100 μm