Fig. 6

ERp57/STAT3/ILF3 feedback loop plays a key role in ccRCC cell proliferation. a SW839 cells were transfected with shILF3 or sh-control then cells were treated with niclosamide or not. A498 cells were transfected with pWPI-ILF3 or empty vector then cells were treated with niclosamide or not. STAT3, p-STAT3, Cyclin E1 and ILF3 protein levels were measured by western blotting. b SW839 cells were treated as (a), BrdU stain was used to test cell viability. Scale bar = 20 μm. Bottom panel showed analysis for BrdU positive cell number. *P < 0.05, vs corresponding control. c SW839 cells were transfected with shILF3 or shSTAT3 alone, or co-transfected with them together. A498 cells were transfected with pWPI-ILF3 or shSTAT3 alone, or co-transfected with them together. Colony formation assay measured cell ability of proliferation. Right panel shows the analysis of colony formation number. *P < 0.05, vs. corresponding control. d SW839 cells were transfected with shILF3 or shERp57 alone, or co-transfected with them together. Cell apoptosis was tested by TUNEL Tunnel apoptosis analysis. Right panel showed analysis for BrdU positive cell number. ** P < 0.01 vs. corresponding control