Fig. 7

Intervention of ILF3/ERp57/stat3 axis inhibits ccRCC xenograft growth in vivo. a SW839 cells engineered to stably knockdown of ERp57, knockdown of ILF3 or knockdown of ERp57 and ILF3 together then the cells were injected subcutaneously to the nude mice to establish ccRCC xenograft tumors. Tumor volumes were monitored by direct measurement. b Representative tumor sizes in each group of mice. c Xenograft tumor wet weight in each group of mice. *P < 0.05, **P < 0.01 vs. corresponding control. d Western blot analysis was used to measure STAT3, p-STAT3, ERp57, ILF3, Cyclin E1 and Cleaved caspase-3 protein level in xenograft tumor. e Tunnel apoptosis analysis was used to test apoptosis in xenograft tumor. Left panel shows the tunnel-positive cell number. *P < 0.05, **P < 0.01 vs. corresponding control. f BrdU stain was used to test cell viability. Scale bar = 20 μm. Right panel showed analysis for BrdU positive cell number. *P < 0.05, ** P < 0.01 vs. corresponding control