Fig. 3

IL-8 knockdown decreased integrin β3 expression and HCC cell invasion and migration. a Real-time PCR analysis of IL-8 mRNA level after 48 h of transfection with IL-8 siRNA or control siRNA (C siRNA). b ELISA analysis of IL-8 protein level after 48 h of transfection with IL-8 siRNA or C siRNA. c Real-time PCR analysis of integrin β3 mRNA level after 48 h of transfection with IL-8 siRNA or C siRNA. d Real-time PCR analysis of integrin αv mRNA level after 48 h of transfection with IL-8 siRNA or C siRNA. e Western blot analysis of integrin β3 expression after 48 h of transfection with IL-8 siRNA or C siRNA. f Western blot analysis of integrin αv expression after 48 h of transfection with IL-8 siRNA or C siRNA. g Invasion assay of HCCLM3 and MHCC97H cells after 48 h of transfection with IL-8 siRNA or C siRNA. h Migration assay of HCCLM3 and MHCC97H cells after 48 h of transfection with IL-8 siRNA or C siRNA. *P < 0.05 compared to cells transfected with C siRNA