Fig. 5

SIRT4 silencing in M2-like TAMs promotes the proliferation, migration, and invasion of HCC cells by enhancing IL-6 production. a-b Overexpression or silencing of SIRT4 in M2-like TAMs inhibited or promoted the migration and invasion of Hepa1–6 cells, respectively, as demonstrated by Transwell assays. c-d Overexpression or silencing of SIRT4 in M2-like TAMs inhibited or promoted the growth of co-cultured Hepa1–6 cells. e Hepa1–6 cells were treated with the supernatant (pre-incubated with IgG or anti-IL-6 neutralizing antibody) from shSIRT-Lv- or Ctr-Lv-infected M2 TAMs. Analysis of cell proliferation after 3 days using CCK-8 assay. f-g Hepa1–6 cells were treated with supernatant (preincubated with IgG or anti-IL-10 (VEGF) neutralizing antibody) from shSIRT-Lv- or Ctr-Lv-infected M2 TAMs. Analysis of cell proliferation after 3 days using CCK-8 assay. h Western blot analysis revealed that SIRT4 was successfully knocked down or overexpressed. *p < 0.05