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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: SIRT4 silencing in tumor-associated macrophages promotes HCC development via PPARδ signalling-mediated alternative activation of macrophages

Fig. 6

SIRT4 silencing in M2-like TAMs promotes M1 macrophage apoptosis by enhancing IL-10 production in HCC peritumour tissues. a-b Investigators characterized the phenotype of apoptotic TAMs (F4/80+) and the apoptotic rate of TAMs by flow cytometry using iNOS (M1) or CD206 (M2). c FCM showed the supernatant of SIRT4 interference in M2-like TAMs on M1 TAM apoptosis. The percentage of early apoptotic cells is located in the lower right quadrant (Annexin V-FITC-positive/PI-negative), and the percentage of late apoptotic cells is located in the upper right quadrant (Annexin V-FITC-positive/PI-positive). d Supernatant of SIRT4 knocked-down M2-like TAMs with the IL-10-neutralizing antibody removed the inhibiting effects of SIRT4 interference in M2-like TAMs on M1 TAM apoptosis. *p < 0.05

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