Fig. 8

Elevated MCP-1 expression induced by SIRT4 silencing is responsible for increased TAM infiltration in HCC peritumour tissues. a The supernatant of SIRT4-knockdown or overexpression HepG2 cells promoted or inhibited the migration of co-cultured THP-1 cells, respectively. b The supernatant of SIRT4-knockdown HepG2 cells with the anti-MCP-1 neutralizing antibody reversed the effects of HepG2 cells on co-cultured THP-1 cells. c The mRNA expression levels of cytokines were determined by qRT-PCR. d Elevated expression of MCP-1 was confirmed in HCC peritumour tissues with low SIRT4 expression. e Effects of SIRT4 on p65 nuclear translocation. Cell nuclei were isolated for western blot analysis. f Suppression of NF-κB induced by its inhibitory ligand reversed the effects of shSIRT4-Lv on MCP-1 expression. * p < 0.05