Fig. 5

USP7 knockdown retarded cell proliferation and induced G2/M cell cycle arrest and chromosome misalignment by targeting PLK1. DU145 and VCaP cells stably expressing control or USP7 shRNAs were transiently transfected with PLK1 plasmid. a and b The same number of cells were seeded for colony formation. A representative experiment out of three independent experiments is shown. Relative numbers of cell colonies were quantified. c The cells were trypsinized and counted at the indicated time points. d DU145 cells were transiently co-transfected with control or USP7 siRNAs together with PLK1 plasmid. Spindles were stained with an immunofluorescent α-tubulin antibody, and nuclear DNA were stained with DAPI. Scale bar, 10 μM. The number of cells with chromosome misalignment were quantified