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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: GSTZ1 deficiency promotes hepatocellular carcinoma proliferation via activation of the KEAP1/NRF2 pathway

Fig. 5

GSTZ1 deficiency promotes HCC cell growth via activation of the KEAP1/NRF2 pathway. a ROS levels in Gstz1-KO1 cells treated with antioxidant NAC for 24 h or NRF2 inhibitor brusatol for 12 h. b-d Effect of NAC or Bru on the proliferation potential of Gstz1-KO1 cells. b Representative images and colony formation potential. c Cell growth curve. d Representative images (left) and enumeration (right) of EdU positive cells. The final concentrations of NAC and brusatol were 4 mM and 40 nM, respectively. Values represent means ± SD (n = 3, performed in triplicate), **p < 0.01, one-way ANOVA test. HCC, hepatocellular carcinoma; ROS, reactive oxygen species

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