Fig. 4

Effects of ALCAR on MMP-9 production in PCa, BPH cell lines and TNFα pre-activated HUVEC cells. PCa (PC-3, DU-145), BPH cell lines and TNFα pre-activated HUVEC cell were pre-treated for 24 h with ALCAR 1 and 10 mM and tested for the ability to produce MMP-9 by flow cytometry. Bar histograms and representative contour plots showing that ALCAR was able to limit MMP-9 production in PC-3, DU-145, BPH cell lines, and TNFα pre-activated HUVEC cell (a, b, c and d). Blockade of MMP-9 production by ALCAR was statistically significant in DU-145 and BPH cell lines. TNFα pre-activated HUVEC cell had a significant reduction in MMP-9 production upon ALCAR treatment. Results are showed as normalised over non treated cells (0), as mean ± SEM, ANOVA, ***p < 0.001, ****p < 0.0001